Multichamber Microfluidics System for Biochemistry and Molecular Biology

 

 

 

In order to study the interaction of biomolecules, cells and microfluidics, we used a multichamber system. A single cell can be cultured in several microfluidic chambers. This method is commonly referred to as "microfluidics" and has a number of advantages. It is a highly flexible platform, which is suitable for biomolecular analysis. Moreover, the devices can be used in a variety of research applications.

 

For biochemistry and molecular biology, we used a microfluidics chamber for a single cell culture. Using this system, we were able to isolate the cytokinesis protein expression in different cell types. We injected the cells into a small compartment of the microfluidics chamber and fixed them using 3% paraformaldehyde-in-HBSS for 10 min. Then, we attached coverslips to the sticky-Slide VI 0.4 with poly-l-ornithine. Then, we mounted the fluorescently-labeled cells in the somatodendritic compartment of the chamber overnight at 4degC.

 

The microfluidics chambers can be customized to meet the requirements of the experimental design. The size of the service microchannels allows the individual treatment of each subcellular compartment. The treatments can be either continuous or pulsatile, and can be customized to the concentration required. The microfluidics chambers are small enough for high-throughput studies and can be compatible with the set-up of microscopy. view here for further insight on this topic.

 

One of the most important features of microfluidics is the ability to control the fluidic interface and molecular gradients. In a single experiment, Shi et al. created a hybrid microfabricated Campenot chamber and investigated the interaction of fibroblast growth factor receptors and the adhesion protein N-cadherin. In the second chamber, the neuron extensions passed through a microchannel barrier.

 

The microfluidics chambers enable the manipulation of fluidic interfaces and molecular gradients, which in turn allow scientists to create a controlled microenvironment. According to a recent study by Shi and colleagues, a hybrid microfabricated Campenot chamber enables researchers to study the effect of the fibroblast growth factor receptor and the adhesion protein N-cadherin on a cell's activity.

 

A microfluidics chamber allows researchers to isolate and treat subcellular compartments of interest. This is particularly useful for research in Alzheimer's disease. Xona Microfluidics also has a unique, open-chamber neuron culture device. Visit xonamicrofluidics.com to learn more about microfluidics chambers. The Innsbruck chamber consists of a silicone-based chamber with two open outer compartments. During the study, the company places round microfluidic chambers in 12-well plates.

 

The KG1a cells were rolled on the E-selectin-coated microfluidic chamber. The E-selectin-coated chamber was then fixated for 90 seconds. The result of the fixation was a clear view of the KG1a cells. These results were obtained through a wide range of experiments. However, this technique has some limitations. It has not been widely applied in clinical settings.

Check out this related post to get more enlightened on the topic: https://www.britannica.com/technology/microfluidic-system.